Scientists have made a new discovery about age-related macular degeneration

Summary: Treatment with Humanin G decreased protein levels of inflammatory markers, which increase with age macular degeneration.

A source: Impact Journals

Inflammatory processes contribute to the development of age-related macular degeneration (AMD), a major cause of vision loss in the United States.

This is new arms In the study, researchers at the University of California, Irvine and the University of Southern California compared protein levels of inflammatory markers in normal and AMD retinal pigment epithelium (RPE) transmitchondrial hybrid cells and studied the effects of treatment with exogenous Humanin G.

Humanin G (HNG) is a peptide derived from mitochondria that is a cytoprotector in AMD and can protect against mitochondrial and cellular stress caused by damaged AMD mitochondria.

“The aim of this study was to test the hypothesis that inflammatory marker-related marker protein levels increase in AMD and that treatment with HNG leads to a decrease in their protein levels.”

Humanin G protein levels were measured in the plasma of patients with AMD and in normal subjects using ELISA analysis. Humanin G was added to normal (control) hybrids obtained from AMD and clinically characterized AMD patients and normal (control) subjects.

Cell lysates were obtained from AMD and conventional hybrids treated with untreated and HNG and Luminex XMAP multiplex analysis was used to measure the level of inflammatory proteins.

The researchers found that there was a differential level of inflammatory proteins between normal and AMD plasma samples. Compared with control plasma samples, AMD showed higher protein levels of plasma inflammatory markers.

Inflammatory processes contribute to the development of age-related macular degeneration (AMD), a major cause of vision loss in the United States. Image in public domain

However, plasma endogenous humanin protein levels were 36.58% lower in patients with AMD than in normal subjects. After treatment with Humanin G, the researchers found a significant decrease in protein levels of increased inflammatory markers in AMD RPE transmitochondrial hybrid cells.

“In conclusion, we propose new findings: a) decreased levels of humanin protein in AMD plasma. Normal plasma; b) suggest the role of inflammatory markers in AMD pathogenesis and c) emphasize the positive effect of Humanin G in reducing inflammation in AMD.

According to the teams, this is the first study to show humanin protein levels in patients with AMD, thus confirming Humanin’s key role in maintaining tissue homeostasis and normal eye function.

“Our discovery is new and AMD can contribute to the development of therapeutic tools to reduce inflammation to alleviate the pathology of the disease,” the researchers concluded.

This is the news of age-related macular degeneration research

Author: Press service
A source: Impact Journals
The connection: Press Service – Impact Journals
Photo: Image in public domain

Original study: Open access.
“Inflammatory effects of Humanin G (HNG) on age-related macular degeneration (AMD)” Sonali Nashine et al. arms

See also

This represents a cluster of neurons activated by FEzf2

Abstract

Inflammatory effects of Humanin G (HNG) on age-related macular degeneration (AMD)

Inflammation plays a key role in the etiology and pathogenesis of AMD (age-related macular degeneration). Humanin G (HNG) is a mitochondrial derivative peptide (MDP) that is a cytoprotector in AMD and can protect against mitochondrial and cellular stress caused by damaged AMD mitochondria.

The aim of this study was to test the hypothesis that marker protein levels associated with inflammation increase in AMD and that treatment with HNG leads to a decrease in their protein levels. Human protein levels were measured in the plasma of AMD patients and in normal subjects using ELISA analysis. Humanin G was added to AMD and normal (control) hybrids, whose nuclei were mitochondrial insufficient ARPE-19 cells, but differed in clinical characteristics from AMD patients and in the content of mitochondrial DNA (mtDNA) obtained from normal (control) subjects.

Cell lysates were obtained from AMD and conventional hybrids treated with untreated and HNG and Luminex XMAP multiplex analysis was used to measure the level of inflammatory proteins. AMD showed a decrease in plasma levels of humanin protein, but the protein level of inflammatory markers was higher compared to control plasma samples.

Decreased levels of Humanin G CD62E / E-Selectin, CD62P / P-Selectin, ICAM-1, TNF-α, MIP-1α, IFN-γ, IL-1β, IL-13 and IL-17A in AMD RPE hybrid cells and Humanin G suggests that it can save inflammation from mtDNA mediation in AMD hybrids.

In conclusion, we propose new findings: A) AMD plasma levels of humanin protein decreased. normal plasma; B) suggests the role of inflammatory markers in the pathogenesis of AMD and C) emphasizes the positive effects of Humanin G in reducing inflammation in AMD.

Leave a Comment

Your email address will not be published.