Brain drainage system in 3D

Summary: The researchers found that the CSF drainage pathways are similar between mice and humans.

A source: Yale

Meningeal lymphatic vessels are potential targets for the treatment of brain diseases. Laboratories at Yale and the Brain Institute of Paris (Hospital Petit-Salpetriere, Paris) have described brain drainage through the meningeal lymphatics in mice and humans.

Near Journal of Experimental Medicine Jean-Leon Thomas, Ph.D., professor of neurology, and Anne Eichmann, Ph.D., associate professor of medicine and professor of cellular and molecular physiology and co-director of the Yale Cardiovascular Research Center (YCVRC), CSF drainage pathways in mice and humans shows similar and represents a new MRI-based imaging technique for patients with neurological diseases.

The lymphatic system controls the immune system and the removal of waste products from tissues and organs. Lymphatic vessels are not located in the central nervous system (CNS), but are located at the borders of the CNS, in the meninges that protect the brain and spinal cord. Meningeal lymphatic vessels drain the lymph nodes of the neck and the peripheral immune system, making them key players in the immune control of the brain.

Meningeal lymphatic vessels are also important for the removal of waste products from the brain by participating in the clearance of interstitial fluid and soluble proteins, as well as participating in CSF drainage, which provides a fluid buffer that protects the brain against injury. nutrient and cellular waste disposal system.

The meningeal lymphatic system affects many neurological diseases in mouse models, including Alzheimer’s disease, multiple sclerosis, brain tumors, and other conditions. “Because of its involvement in many diseases, the meningeal lymphatic system has attracted therapeutic interest,” explains Laurent Jacob, Ph.D., first author of the study and member of the Paris study team.

“However, it remains unclear where lymphatic retention of CSF molecules occurs in the context of the whole head, in mice or humans.”

To learn more about the architecture and function of the meningeal lymphatic network, the team studied CSF lymphatic drainage in mice using postmortem light sheet and real-time magnetic resonance imaging. By combining these methods, the authors reconstructed the entire lymphatic drainage network of the CSF.

The 3D image showed that the meningeal lymphatics were in communication with the venous sinuses of the dura mater and revealed an extensive meningeal lymphatic network around the cavernous sinus in the anterior part of the skull. From there, the meningeal lymphatic vessels exit the skull through the cranial foramen and drain into the cervical lymph nodes.

Meningeal lymphatic vessels are potential targets for the treatment of brain diseases. Photo courtesy of researchers

Dr. Stephanie Lenk of the Pitier-Salpetriere Hospital performed quantitative lymphatic MRI on 11 patients with various neurological disorders. He created a procedure for 3D visualization of the meninges and all the blood and lymphatic vessels in the neck, which revealed a significantly higher meningeal lymph volume in men than in women.

Future studies should investigate whether these anatomical features are associated with a greater susceptibility of women to develop neurological diseases such as multiple sclerosis, meningioma, or intracranial hypertension.

“Meningeal lymphatic vessels are a potential target for the treatment of brain diseases,” Eichmann said. “Laboratories at Yale are making progress in determining their function by imaging brain drainage through meningeal lymphatic vessels in mice and humans.”

Neuroscience research news about it

Author: Elizabeth Reitman
A source: Yale
The connection: Elizabeth Reitman – Yale
Photo: Photo courtesy of researchers

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Original research: Open access.
“Conserved Meningeal Lymphatic Drainage Patterns in Mice and Humans” by Laurent Jacob et al. Journal of Experimental Medicine


Conserved patterns of meningeal lymphatic drainage in mice and humans

Meningeal lymphatic vessels (MLVs) have been identified in the dorsal and caudobasal regions of the dura mater, where they provide waste disposal and immunosurveillance of brain tissue. Whether MLVs are present in the anterior cranium of the mouse and human and how they relate to the glymphatic and extracranial lymphatic systems remains unclear.

Here, we used light-sheet fluorescence microscopy (LSFM) of mouse whole-head preparations after OVA-A.555 real-time magnetic resonance imaging (VW-MRI) of the vessel wall (VW) was performed after tracer injection into the cerebrospinal fluid (CSF) and systemic gadobutrol injection in patients with neurological pathology.

We observed a conserved three-dimensional anatomy of MLVs in mice and humans, corresponding to dural venous sinuses but not nasal CSF outflow, and we found an extended anterior MLV network around the cavernous sinuses with emissary venous exits. VW-MRI may provide a diagnostic tool for patients with CSF drainage defects and neurological disorders.

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